# Metabolic comparison of minimally to noninvasive urogenital sample types for studying gynecologic health: A pilot study

## TL;DR

This pilot study compares metabolites in different urogenital samples to understand gynecologic health better.

## Contribution

The study reveals unique metabolic profiles in minimally invasive gynecologic biospecimens.

## Key findings

- CVLs show amino acid signatures like polyamines in dysbiosis.
- Urine reveals estrogenic steroids and endocrine-disrupting compounds.
- Vaginal swabs highlight lipid signatures.

## Abstract

In this pilot study, we investigated three minimally to noninvasive biospecimens: cervicovaginal lavages (CVLs), vaginal swabs, and urine, from 18 premenopausal women using untargeted metabolomics to inform future gynecologic research. Metabolic profiles were compared between specimen types and between participants grouped based on vaginal microbiome composition and body mass index (BMI). We identified metabolites altered in the groups in all sample types; however, different metabolites were uniquely detected in each biospecimen. Within CVLs, key amino acid signatures (i.e., polyamines) were identified in women with dysbiosis. While polyamines were also detected in vaginal swabs, this sample type exhibited more lipid signatures. In urine, we specifically detected estrogenic steroids, endocrine-disrupting compounds, and certain drugs. Overall, each urogenital biospecimen type exhibits unique metabolic profiles and, based on metabolic alteration in the context of vaginal microbiome and BMI, can be valuable tools in answering questions related to gynecologic health.

•Each biospecimen detects distinct metabolites, offering complementary insights•Urine provides unique insights into the systemic macroenvironment•CVLs provide greater information on vaginal biogenic amines•Vaginal swabs provide deeper information on vaginal lipid profiles

Each biospecimen detects distinct metabolites, offering complementary insights

Urine provides unique insights into the systemic macroenvironment

CVLs provide greater information on vaginal biogenic amines

Vaginal swabs provide deeper information on vaginal lipid profiles

Women’s health; Microbiome; Metabolomics