# Extracellular vesicles aid in the transfer of long-term associative memory between Caenorhabditis elegans

## TL;DR

This study shows that memory can be transferred between nematodes using extracellular vesicles, which carry learned associations between smells and heat.

## Contribution

The paper introduces extracellular vesicles as a novel mechanism for transferring long-term associative memory in nematodes.

## Key findings

- Extracellular vesicles released during training cause loss of attraction to IAA in C. elegans.
- Vesicles are specific to the training cues and can transfer memory between species.
- The memory transfer mechanism is conserved between C. elegans and C. briggsae.

## Abstract

Memory formation is necessary for the survival of animals across phyla. Here, we elucidate the mechanism underlying the formation of long-term associative memory (LTAM) formed by treating Caenorhabditis elegans with a volatile chemoattractant and heat. Previous work has shown that training animals with a paradigm involving heat and isoamyl alcohol (IAA) simultaneously, causes C. elegans to lose their attraction to IAA. In this study, we elaborate on the mechanism behind this LTAM formation and suggest that during training with heat and IAA, C. elegans release extracellular vesicles (EVs) that upon being taken up by the same trained animals or their untrained counterparts causes the organism to lose attraction to IAA. Our data suggests that the vesicles are highly specific to the training paradigms used and differ with differing cues. Finally, we show that this mechanism of transfer of LTAM appears to be conserved between C. elegans and C. briggsae allowing for both intra and interspecies transfer of memory.