# Complete genome sequence data of Xylella fastidiosa subspecies multiplex ST88 and ST89 indicate distinct introductions in France

## TL;DR

This paper reports the complete genome sequences of two new Xylella fastidiosa strains in France, helping to understand their origin and diversity.

## Contribution

The study provides complete genome sequences and phylogenetic analysis of two new Xylella fastidiosa subspecies multiplex strains (ST88 and ST89) in France.

## Key findings

- ST88 and ST89 strains were isolated from infected plants in Provence-Alpes-Côte d’Azur.
- Genome assemblies were created using PacBio and Illumina sequencing data.
- Phylogenomic analysis revealed distinct introductions of these strains in France.

## Abstract

Xylella fastidiosa is a Gram-negative bacterium native to the Americas and classified as a priority pest under EU regulations. This xylem-limited plant pathogenic bacterium has a wide host range and is transmitted by insect vectors. Since 2013, X. fastidiosa has been identified in several European countries including Italy, France, Spain and Portugal, with different subspecies and sequence types (ST) detected. Since 2015, most strains identified in France are of the subspecies multiplex, specifically ST6 and ST7. Two new STs of X. fastidiosa subsp. multiplex, ST88 and ST89, were recently detected in the region Provence-Alpes-Côte d’Azur (PACA), and one strain of each ST has been isolated from infected plants. To investigate the phylogenetic relationships between the four STs present in France, a complete circular genome and a single-contig genome were assembled for the ST89 and ST88 strains, respectively, by combining PacBio and Illumina sequencing data. A phylogenomic analysis was performed to investigate the phylogenetic position and potential origin of these new strains. This data article contributes to improve our knowledge of the diversity and origin of X. fastidiosa subsp. multiplex in France and Europe.