# Green first derivative synchronous spectrofluorimetric determination of lacidipine and its acid degradation product in plasma and mixtures

## TL;DR

This study introduces a green analytical method to detect lacidipine and its acid degradation product in plasma and mixtures with high sensitivity and low environmental impact.

## Contribution

A novel first derivative synchronous spectrofluorimetric method is proposed for lacidipine analysis with low health and environmental impact.

## Key findings

- The method is linear in the range of 50–300 ng/mL with a high determination coefficient of 0.9997.
- The limit of detection is 14.51 ng/mL and the limit of quantification is 43.97 ng/mL.
- The method uses a 0.5% Tween-80 solution to enhance fluorescence intensity.

## Abstract

One of the main objectives of this study is to find an analysis method that has a low environmental and health impact and contributes to achieving sustainability. A simple and sensitive method was established for determination of lacidipine in pure active pharmaceutical ingredient, pharmaceutical formulation and spiked human plasma in presence of its acid induced degradation product. The native emission was measured at 430 nm after the excitation at 281 nm. A 0.5% Tween-80 solution was used to enhance fluorescence intensity. Peak amplitude of the first derivative synchronous was measured at 409 nm by using constant wavelength difference (Δλ = λem - λex = costant) which equal 160 nm. The regression plot of the proposed first derivative synchronous spectrofluorimetric method was found to be linear over the range of 50–300 ng/mL with a determination coefficient equals 0.9997, limit of detection (LOD) was 14.51 ng/mL but limit of quantification (LOQ) was 43.97 ng/mL.

The online version contains supplementary material available at 10.1038/s41598-025-11341-y.