# Mass spectrometry dataset of conventional and organic tempe before and after in vitro digestion

## TL;DR

This study uses mass spectrometry to compare the metabolite profiles of organic and conventional tempe before and after digestion.

## Contribution

The study provides a novel dataset comparing organic and conventional tempe metabolomes before and after digestion.

## Key findings

- Metabolomic profiles of soybean, tempe, and digested tempe were characterized using UHPLC-HRMS.
- The dataset includes detailed information on polarity, m/z, retention time, and biological replicates.
- The data reveals preliminary insights into the fate of bioactive metabolites during digestion.

## Abstract

Tempe is a superior plant-based protein source that provides a diverse array of nutritional benefits as a result of the presence of bioactive metabolites. Nevertheless, there is a scarcity of information regarding the metabolomics profile between organic and conventional tempe and the fate of these metabolites after in vitro digestion. This report examines the metabolomic profile of soybean as raw material and tempe prior to and following the in vitro digestion process. We obtained a comprehensive set of metabolomic data using ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS). The metabolomics dataset organized into Excel sheets and structured according to polarity, mass to charge ratio (m/z), retention time, feature name, biological replicates and controls. This data offers preliminary insights into the metabolite profile of tempe samples, encompassing source material soybean, tempe, and tempe digesta.