# Conductive Hairy Particles With Homogeneous and Janus Design as Carrier Materials for the Efficient Immobilization of Unspecific Peroxygenases

## TL;DR

Scientists created conductive particles with a special design to efficiently attach enzymes, which could be reused and may help in making biosensors.

## Contribution

The first demonstration of efficient and reusable immobilization of UPOs on conductive Janus hairy particles.

## Key findings

- PDMAEMA brushes with controlled lengths were successfully grown on conductive silver particles.
- MroUPO showed the most efficient immobilization on Janus particles.
- The enzyme and carrier can be reused after cleaning the particles.

## Abstract

Efficient immobilization of unspecific peroxygenases (UPOs) on hairy particles with homogeneous and Janus design possessing conductive core and polymeric shell was demonstrated. PDMAEMA brushes (hairs) with controlled chain lengths were successfully grown from the conductive silver particles allowing further immobilization of enzymes and keeping their activity. The Janus design of the synthesized particles maintained the conductivity of the core material. Enzyme immobilization on brush‐modified particles was first carried out with two laccases as model enzymes [Trametes versicolor (TveLac) and Pycnoporus cinnabarinus (PciLac)] and then successfully extended to UPOs [two wild‐type and one recombinant UPO from Marasmius rotula (MroUPO) and Agrocybe aegerita (AaeUPO, rAaeUPO)]. The most efficient immobilization was achieved for MroUPO on Janus particles. The enzyme loading is reversible and could be successfully repeated after cleaning of the particles. Thus, the successful immobilization of an MroUPO on conductive hairy Janus particles was demonstrated for the first time. The major advantage of the proposed approach lies in the reusability of the enzyme and its carrier, as well as in the use of conductive core materials, which could be promising, for example, as material for electrochemical biosensors in future.

Efficient immobilization of unspecific oxidoreductases on hairy core‐shell particles with homogeneous and Janus design possessing conductive core material and polymeric brush shell is demonstrated. PDMAEMA brushes with controlled chain lengths (4, 11, and 23 nm) are successfully grown from conductive silver particles. Hairy particles with Janus design maintained the conductivity of the hybrid carrier material.