# Live Imaging and Interactome Analysis of Zika and Chikungunya Viral RNAs Via Dual-Action Aptamer Tag

## TL;DR

This paper introduces a new method using a special RNA tag to study how Zika and Chikungunya viruses interact with human cells in real time.

## Contribution

The dual-action Mango-II aptamer tag enables live imaging and interactome analysis of viral RNA in positive-sense RNA viruses.

## Key findings

- The Mango-II aptamer allows high-resolution live imaging of flavivirus RNA in infected cells.
- Over 1,000 host interactors were identified for Zika and Chikungunya viral RNA.
- Spliceosome factor SFPQ was found to decrease RNA production in both viruses when depleted.

## Abstract

Arthropod-borne, positive-sense RNA viruses encompass many human pathogens posing significant health threats worldwide. Understanding intracellular dynamics and host-factor interactions of the viral genome is essential for devising effective antiviral strategies. We engineered dual-labeled Zika and chikungunya viruses incorporating a Mango-II aptamer for RNA pulldown and real-time imaging, achieving high-resolution imaging of flavivirus RNA in live cells. Tandem imaging with viral replication proteins revealed intracellular dynamics of replication complexes and translating RNA of these viruses. We identified > 1,000 high-confidence host interactors of viral RNA and corroborated 355 hits from existing data sets. Spliceosome factor SFPQ was identified as a common interactor decreasing RNA production in both viruses when depleted. Intracellular colocalization of SFPQ-ZIKV RNA was confirmed through super-resolution imaging of infected cells. Thus, we demonstrate Mango-II aptamer tagging as an innovative strategy for identifying spatiotemporal dynamics and virus-host interactions of viral genomes in positive-strand RNA viruses.