# Generation of six4 -nlsRFP: a red somatic gonadal nuclear marker for live imaging Drosophila gonadogenesis

## TL;DR

Researchers created a red fluorescent marker to track cell movements in fruit fly gonad development using live imaging.

## Contribution

A new red fluorescent nuclear marker for somatic gonadal precursor cells in Drosophila was developed for live imaging.

## Key findings

- A red fluorescent nuclear marker was generated for live tracking of somatic gonadal precursor cells.
- The marker enables visualization of cell interactions during Drosophila gonadogenesis.
- The tool complements existing green fluorescent reagents for cytoskeletal and adhesion studies.

## Abstract

Intrinsically fluorescent tissue labels are valuable to live image cell behaviors during development. Fluorescent markers of
Drosophila
gonadal cells have elucidated dynamic cellular interactions that shaped our conception of cell biology. Many green fluorescent protein (GFP) reagents are available to visualize cytoskeletal dynamics, extracellular matrix, and cell adhesion proteins. To facilitate use of these reagents while studying somatic gonadal development, we generated a somatic gonadal precursor (SGP) intrinsic red fluorescent nuclear marker. This tool can track SGP movement live, facilitating the study of somatic cell interactions required for gonadogenesis.