# Effects of “Living High‐Training Low and High” on Sleep, Heart Rate Variability, and Psychological Responses in Elite Female Cyclists

**Authors:** Thibaud Pirlot, Thibaud Mihailovic, Philippe Gimenez, Gregoire P. Millet, Franck Brocherie, Eric Fruchart, Gilles Ravier, Bertrand Baron, Romain Bouzigon, Sandrine Guirronnet, Emmanuel Brunet, Alain Groslambert

PMC · DOI: 10.1002/ejsc.12320 · 2025-07-15

## TL;DR

This study explores how a high-altitude training method affects sleep, heart rate, and stress in elite female cyclists.

## Contribution

It provides the first insights into psychophysiological stress in female athletes using the LHTLH training method.

## Key findings

- Early acclimatization to LHTLH causes sleep disturbances and altered heart rate variability.
- Restorative sleep returns after 4 days, but HRV and stress remain impaired for at least 10 days.
- Athletes can increase training load after initial acclimatization despite ongoing physiological stress.

## Abstract

“Living High‐Training Low and High” (LHTLH) is an altitude/hypoxic training method used to improve physical performance at sea level. The aim of this exploratory study was to investigate the effects of LHTLH on sleep, heart rate variability (HRV), and psychological stress in 10 elite/international level female cyclists (mean age: 17.3 ± 1.2 years). Participants were monitored for 19 days divided into 3 periods: (i) normoxia (5 days preceding LHTLH), (ii) early acclimatization (day 1–4 of LHTLH), and (iii) middle acclimatization (day 5–14) performed in hypoxic rooms (FiO2 = 15.09%). Questionnaires of psychological stress and sleep disturbance, sleep architecture (determined through an electroencephalography sleep headband), and HRV (measured at rest with a chest strap) were recorded during the 3 periods. The results found that, compared to normoxia, there was no significant difference in total sleep time, sleep efficiency, latency, or waking during the early acclimatization period. However, a significant increase in sleep disturbance (2.5 ± 1.1 vs. 4.9 ± 2.5 a.u. and p < 0.001), alterations of HRV, and sleep architecture with a significant increase in stages 1 (21.8 ± 3 vs. 25.9 ± 3.6 min and p < 0.007) and 2 (201.2 ± 55 vs. 238.5 ± 55 min and p < 0.008) of sleep was observed. During the middle acclimatization period, the athletes had restorative sleep but HRV remained altered, with a significant increase in external tension (1.24 ± 0.4 vs. 2.83 ± 1.8 a.u. and p < 0.05). All these findings suggest that an acclimatization period of at least 4 days is required to recover restorative sleep during LHTLH intervention.–This article provides the first insights on the understanding of psychophysiological stress induced using the “Live High‐Training Low and High” paradigm, particularly in elite/international level female athletes.–Results indicate that:during the early acclimatization period, sleep disturbances are observed, with an alteration in heart rate variability.After this period, the athletes return to restful sleep, enabling them to increase their training load.However, heart rate variability and psychological stress remain impaired for at least 10 days.

This article provides the first insights on the understanding of psychophysiological stress induced using the “Live High‐Training Low and High” paradigm, particularly in elite/international level female athletes.

Results indicate that:during the early acclimatization period, sleep disturbances are observed, with an alteration in heart rate variability.After this period, the athletes return to restful sleep, enabling them to increase their training load.However, heart rate variability and psychological stress remain impaired for at least 10 days.

during the early acclimatization period, sleep disturbances are observed, with an alteration in heart rate variability.

After this period, the athletes return to restful sleep, enabling them to increase their training load.

However, heart rate variability and psychological stress remain impaired for at least 10 days.

## Full-text entities

- **Diseases:** sleep disturbance (MESH:D012893), hypoxic (MESH:D002534)
- **Species:** Homo sapiens (human, species) [taxon 9606]

## Figures

3 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12262107/full.md

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Source: https://tomesphere.com/paper/PMC12262107