Unveiling the hemolymphatic miRNome composition of the schistosomiasis vector snail Biomphalaria glabrata

TL;DR

This study identifies and characterizes microRNAs in the immune compartment of a snail that transmits schistosomiasis, highlighting their potential role in immune regulation.

Contribution

The discovery of 25 novel miRNAs and their potential role in regulating immune genes in Biomphalaria glabrata snails.

Findings

63 miRNAs, including 25 novel ones, were identified in the hemolymph of Biomphalaria glabrata.

Over 6000 potential miRNA target genes were predicted, including BgTEP1, a complement-like factor involved in parasite clearance.

A newly identified miRNA, bgl-miR-22707-5p, was found to target BgTEP1.

Abstract

MiRNAs are single-stranded short noncoding sequences which display crucial roles on gene transcription regulation in many biological processes especially such as embryonic development, cell proliferation or apoptosis. Also, they are recognized for triggering the host’s internal defence mechanisms and immune cell responses thereby playing crucial role in host-parasite interactions. In the present study, a snap-shot of miRNAs, referred to as the miRNome, is described from the hemolymph, the main immune-related compartment of Biomphalaria glabrata snails, one of the intermediate hosts of the trematode parasite Schistosoma mansoni, the causative agent of schistosomiasis. A high throughput sequencing approach of small RNAs has revealed the presence of 63 miRNAs in the hemolymphatic compartment. Mollusc-specific miRNAs including bgl-miR-1985-5p and bgl-miR-1984-5p were identified, along with 25 novel miRNAs. Bioinformatic predictions, thanks to multiple software tools, helped us to identify more than 6000 potential miRNA target gene candidates. Among them is BgTEP1, a complement-like factor involved in parasite clearance. Interestingly, this factor appeared to be targeted by a newly identified miRNA, named bgl-miR-22707-5p. Our study underscores the inherent diversity of miRNAs in the hemolymph of B. glabrata and discusses their potential role in the regulation of the snail’s innate immune response.

Image 1

•Constitutive hemolymphatic microRNAs from Biomphalaria glabrata.•Characterization of 25 new miRNAs over 63 yet identified.•More than 6000 potential miRNA target gene candidates identified.•MicroRNAs potential regulation of immune genes.

Constitutive hemolymphatic microRNAs from Biomphalaria glabrata.

Characterization of 25 new miRNAs over 63 yet identified.

More than 6000 potential miRNA target gene candidates identified.

MicroRNAs potential regulation of immune genes.

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