# Hsa_circ_0001859 promotes NSCLC progression through the miRNA-101-3p/MMP1 axis

## TL;DR

This study shows that a circular RNA called circ_0001859 helps lung cancer grow and spread by interacting with miR-101-3p and MMP1.

## Contribution

The study is the first to investigate the specific role of circ_0001859 in NSCLC and its regulatory mechanism.

## Key findings

- Circ_0001859 is overexpressed in NSCLC tissues and cells.
- Silencing circ_0001859 reduces NSCLC cell proliferation, migration, and invasion.
- Circ_0001859 acts as a sponge for miR-101-3p to modulate MMP1 expression in NSCLC.

## Abstract

Non-small cell lung cancer (NSCLC) is a prevalent form of lung cancer characterized by a significant incidence and mortality rate in China. Most patients are diagnosed at an advanced stage. Circ_0001859 served as an exon circular RNA, but its specific role in NSCLC remained extensively unexplored.

Quantitative Real-Time-Polymerase Chain Reaction (qRT-PCR) and western blotting were utilized to detect messenger RNA (mRNA) and protein expression levels in NSCLC tissues and cells. Cell proliferation was assessed by Cell Counting Kit-8 (CCK-8) and colony formation assays. Cell migration and invasion were evaluated by transwell assay. Mechanistically, the mechanisms and target binding relationship between circ_0001859, miR-101-3p and MMP1 were assessed through the luciferase reporter and RNA immunoprecipitation (RIP) assays. In vivo xenograft model was established to examine the impact of circ_0001859.

Circ_0001859 was significantly overexpressed in NSCLC tissues and cells. Functional studies demonstrated that silencing circ_0001859 significantly impeded the malignant phenotype of NSCLC cells. Bioinformatics analysis and rescue experiments disclosed that circ_0001859 functioned as a sponge for miR-101-3p, modulating MMP1 expression and thereby controlling NSCLC development and metastasis. The role of circ_0001859/miR-101-3p/MMP1 axis was validated in xenograft tumor models in vivo.

Our research findings demonstrated that circ_0001859 engaged in regulating NSCLC growth and metastasis via the miR-101-3p/MMP1 pathway. These studies presented the first investigation into the precise function and putative regulatory mechanism of circ_0001859 in NSCLC, providing valuable insights towards prognostic indicators and therapeutic targets for malignant tumors.