Antibacterial potential and chromatographic profiling of bioactive compounds from endophytic Streptomyces sp. strain MIRK71 isolated from Mirabilis jalapa (L.)
Lalrokimi, Purbajyoti Deka, William Carrie, Lallawmsangi, Christine Vanlalbiakdiki Sailo, Lalrosangpuii, Felicia Lalremruati, Awmpuizeli Fanai, Yasangam Umbon, Esther Lalnunmawii, Zothanpuia
TL;DR
This study explores the antimicrobial potential of a Streptomyces strain from Mirabilis jalapa, identifying bioactive compounds that could help combat drug-resistant bacteria.
Contribution
The study identifies a potent Streptomyces strain and its bioactive compounds with antimicrobial activity against multidrug-resistant pathogens.
Findings
Strain MIRK71 showed the strongest antimicrobial activity among 18 tested endophytic actinobacteria.
GC-MS analysis identified 20 volatile compounds in the antimicrobial extract.
HPTLC profiling revealed distinct peaks for glycosides, phenols, and anthracenes in optimized solvent systems.
Abstract
Multidrug-resistant bacteria pose an alarming global health threat. The persistent rise in antibiotic-resistant infections continues to challenge healthcare systems worldwide. This study aims to evaluate the antimicrobial potential of 18 endophytic actinobacteria isolated from Mirabilis jalapa (L.) against clinically relevant multidrug-resistant pathogens. Among these, strain MIRK71 exhibited the strongest activity in secondary antimicrobial screening and was selected for further investigation. Molecular characterization using 16S rRNA gene sequencing identified MIRK71 as a Streptomyces species. Morphological analysis via scanning electron microscopy (SEM) revealed branched, filamentous colonies with microspore chains. Antimicrobial compounds were extracted from the culture filtrate grown in ISP1 broth using methanol under reduced pressure in a rotary evaporator. Gas chromatography-mass…
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Taxonomy
TopicsMicrobial Natural Products and Biosynthesis · Mycobacterium research and diagnosis · Essential Oils and Antimicrobial Activity