CD8+ T cells promote tubule-interstitial damage in malaria-induced acute kidney injury

TL;DR

This study shows that CD8+ T cells contribute to kidney damage in malaria patients, offering new insights into the disease's progression and potential treatments.

Contribution

The study identifies CD8+ T cells as key drivers of tubule-interstitial injury in malaria-induced kidney damage.

Findings

Transferring T cells from infected mice to healthy ones caused kidney tubule damage without affecting glomerular function.

Depleting CD8+ T cells in infected mice protected against tubular injury but not glomerular damage.

FTY720 treatment reduced markers of tubular injury without affecting glomerular markers.

Abstract

Malaria acute kidney injury (MAKI) is associated with severe malaria and correlates with poor prognosis and death of patients infected with Plasmodium falciparum. The pathogenesis of MAKI is not completely understood but some hypotheses are well recognized. Host–parasite interactions lead to mechanical obstruction, disorders in the renal microcirculation, and immune-mediated glomerular injury. We investigated the influence of CD8⁺ T cells in the pathogenesis of malaria-induced renal disease.

To assess the role of T lymphocytes in MAKI pathogenesis, we used adoptive transfer; antibody-driven CD8+ T cells depletion and treatment with FYT720.

The transference of total T cells isolated from malaria-infected donor mice into naive recipient animals reproduced kidney tubule-interstitial damage without affecting glomerular function. It was associated with increased accumulation of CD8+ T cells in the kidneys of recipient mice. The selective depletion of CD8+ T cells in infected animals resulted in protection from tubular injury, although glomerular alterations still occurred. Finally, we evaluated FTY720, an immunomodulatory drug that sequesters T cells in lymphoid organs and limits their migration, as a potential therapeutic strategy. Treatment with FTY720 prevented the development of proteinuria and the increase in the urine to creatinine ratio. Moreover, FTY720 reduced urinary γ-glutamyl transferase (γ-GT) levels, a marker of tubular injury, but did not alter plasma urea and creatinine, two markers of glomerular function.

Our results add new knowledge demonstrating that CD8+ T cells have a specific role in tubule-interstitial injury pathology during MAKI.

Figures