Screening of host proteins interacting with the African swine fever virus outer membrane protein CD2v

TL;DR

This study identifies host proteins that interact with a key African swine fever virus protein, revealing how the virus may manipulate host cells to aid its replication.

Contribution

The study identifies novel host proteins interacting with ASFV's CD2v protein and their roles in viral pathogenesis.

Findings

Host proteins interacting with CD2v are involved in translational regulation, inflammation, immune signaling, and iron metabolism.

Functional analyses suggest ASFV influences host cell functions through multiple pathways to support viral replication.

Interaction network analysis provides new insights into the pathogenic mechanisms of ASFV.

Abstract

The African swine fever virus (ASFV) is a highly pathogenic double-stranded DNA virus that poses a significant threat to the global swine industry. Although the CD2v protein encoded by ASFV is a key factor in viral immune evasion and pathogenicity, the mechanism underlying its interaction with host proteins remains unclear.

The aim of this study was to identify a range of potential host proteins that interact with the CD2v protein using the membrane yeast two-hybrid technology.

Through subsequent validation experiments and functional analyses, we discovered that these proteins are involved in critical cellular processes such as translational regulation, inflammatory responses, immune signaling, and iron metabolism. Furthermore, interaction network and functional enrichment analyses revealed that ASFV might influence host cell functions through multiple pathways to facilitate viral replication.

This study provides new insights into the pathogenic mechanisms of ASFV and offers valuable clues for identifying antiviral targets.

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