# Genome-wide characterization of the GmLUX binding preferences and its epigenic features in the soybean genome

**Authors:** Lv Tianxiao, Hong Xiao, Jingxing Wang, Ling Zhang, Tian Fan, Mingkun Huang, Chang-En Tian, Hua Yang, Yufang Hu

PMC · DOI: 10.3389/fpls.2025.1607224 · 2025-06-30

## TL;DR

This study explores how the GmLUX protein regulates flowering in soybeans by analyzing its genome-wide binding and epigenetic features.

## Contribution

The study identifies a new regulatory pathway of GmLUX by showing its direct regulation of a CO-like gene through chromatin interactions.

## Key findings

- GmLUX binding sites were characterized genome-wide using ChIP-seq and other sequencing methods.
- GmLUX facilitates chromatin interactions to regulate a CO-like gene involved in flowering.
- The findings provide genomic resources for understanding GmLUX's regulatory mechanisms.

## Abstract

Transcription factors function in complex regulatory networks to regulate various biological and physiological processes. In soybean (Glycine max), GmLUX, as an important component of the evening complex, plays a critical role in the regulation of soybean flowering regulation. In this study, the genome-wide characterization and epigenetic features of GmLUX binding sites have been analyzed using high-throughput sequencing methods, such as ChIP-seq, Hi-C, histone modification and ATAC-seq. In addition, combined with molecular experiments, GmLUX was found to be able to directly regulate the CO-like gene by facilitating chromatin interactions, suggesting a new regulatory pathway of GmLUX in controlling flowering, which provided the important genomic resources for a further understanding of its regulatory mechanism.

## Linked entities

- **Genes:** LOC137823361 (zinc finger protein CONSTANS-LIKE 2-like) [NCBI Gene 137823361]
- **Species:** Glycine max (taxon 3847)

## Full-text entities

- **Species:** Glycine max (soybean, species) [taxon 3847]

## Figures

5 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12256510/full.md

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Source: https://tomesphere.com/paper/PMC12256510