# BugBuster: a novel automatic and reproducible workflow for metagenomic data analysis

## TL;DR

BugBuster is a new, easy-to-use, and reproducible workflow for analyzing metagenomic data using containers.

## Contribution

BugBuster introduces a modular, containerized, and reproducible metagenomic analysis workflow implemented in Nextflow.

## Key findings

- BugBuster streamlines metagenomic analysis at the level of reads, contigs, and metagenome-assembled genomes.
- The workflow includes dedicated modules for taxonomic profiling and resistome characterization.
- It can be deployed on various platforms with minimal configuration thanks to containerization.

## Abstract

Metagenomic sequencing generates massive datasets that capture the complete genetic content of a sample, enabling detailed characterization of microbial communities. Yet the software and processes necessary to transform raw data into biologically meaningful results have become increasingly complex, limiting accessibility for researchers without specialist expertise. In this work, we present a novel modular a reproducible workflow developed to facilitate the analysis of metagenomic data. BugBuster is a fully containerized, modular, and reproducible workflow implemented in Nextflow. The pipeline streamlines analysis at level of reads, contigs, and metagenome-assembled genomes, offering dedicated modules for taxonomic profiling and resistome characterization. Thanks to the use of containers, BugBuster can be deployed with minimal configuration on workstations, high-performance clusters, or cloud platforms. Together, these features allow the robust, scalable, and reproducible analysis of metagenomic datasets.

BugBuster was written in Nextflow-DSL2. The program applications, user manual, example data and code are freely available at https://github.com/gene2dis/BugBuster.