# Post-mortem Interval Estimation Through Entomology Using Calliphorid Flies: A Case Series

**Authors:** Arwinder Singh, Yashpal. S, Ashish R Bhute, Amit Jangid

PMC · DOI: 10.7759/cureus.85875 · Cureus · 2025-06-12

## TL;DR

This paper discusses using blowflies to estimate time since death in cases with advanced decomposition, offering a reliable method when traditional approaches fail.

## Contribution

The paper introduces a practical method for PMI estimation using forensic entomology in complex decomposition cases.

## Key findings

- Forensic entomology provides reliable PMI estimates in advanced decomposition cases.
- Blowfly colonization patterns and life cycles are key to PMI estimation.
- A straightforward approach is outlined for PMI estimation without direct entomologist access.

## Abstract

Post-mortem interval (PMI) estimation is a critical component of death investigations but becomes particularly challenging in cases involving advanced decomposition, where traditional methods may prove less effective. The combined effects of severe tissue damage, environmental conditions, and interactions with carrion feeders complicate the PMI assessment. Forensic entomology has been utilized to address these challenges by focusing on insect colonization patterns and life cycle analysis. This article emphasizes the value of forensic entomology as an essential tool for estimating the PMI in complex cases involving extensive decomposition, offering a reliable complement to conventional forensic techniques. In addition, this article outlines a straightforward approach for deriving PMI estimates from available data when direct access to a forensic entomologist is not feasible.

## Full-text entities

- **Genes:** Adh (Alcohol dehydrogenase) [NCBI Gene 3771877] {aka Adh3, BG:DS01486.8, CG32954, CG3481, DM-ADH, DmADH}
- **Diseases:** dead (MESH:D001926), Skin loss (MESH:D012871), weight loss (MESH:D015431), death (MESH:D003643), PMI (MESH:D000094025), GDD (MESH:D014786), maggot (MESH:D009198)
- **Chemicals:** volatile organic compounds (MESH:D055549), ethyl (-), isopropyl alcohol (MESH:D019840)
- **Species:** Hydrotaea aenescens (black dump fly, species) [taxon 221905], Atherigona orientalis (pepper fruit fly, species) [taxon 371200], Lucilia sericata (common green bottle fly, species) [taxon 13632], Creophilus maxillosus (species) [taxon 623683], Muscidae (house flies, family) [taxon 7366], Thanatophilus (genus) [taxon 347474], Homo sapiens (human, species) [taxon 9606], Sus scrofa (pig, species) [taxon 9823], Hydrotaea chalcogaster (species) [taxon 1816462], Diptera (flies, order) [taxon 7147], Chrysomya megacephala (oriental latrine fly, species) [taxon 115424], Chrysomya rufifacies (hairy maggot blowfly, species) [taxon 45450], Sarcophaga misera (species) [taxon 321196], Coleoptera (beetles, order) [taxon 7041]

## Full text

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## Figures

13 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12255536/full.md

## References

11 references — full list in the complete paper: https://tomesphere.com/paper/PMC12255536/full.md

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Source: https://tomesphere.com/paper/PMC12255536