# The Effect of Commercially Available Sugar Alternatives on Bacillus Probiotic Viability During Baking

## TL;DR

This study examines how sugar substitutes affect the survival of probiotics during baking, finding that Bacillus strains are more resilient than Lactobacillus strains.

## Contribution

The study provides new insights into the viability of specific probiotic strains under baking conditions with different sugar alternatives.

## Key findings

- Bacillus subtilis showed the highest viability with monk fruit and the lowest with sucralose.
- Lactobacillus acidophilus had the highest log reduction with monk fruit and the lowest with sucralose.
- Bacillus probiotics maintained viability under high-temperature baking, unlike Lactobacillus strains.

## Abstract

Growing health concerns regarding sucrose consumption have led to an increased use of alternative sugars, including sugar alcohols, artificial sweeteners, and natural sweeteners. This study investigated the impact of commercially available sugar substitutes on the viability of Bacillus probiotics and Lactobacillus strains during baking. Our findings revealed that Bacillus subtilis strains exhibited the highest log reduction with sucralose (average 0.99 log CFU/g) and the lowest with monk fruit (average 0.60 log CFU/g). In contrast, Lactobacillus acidophilus demonstrated a higher log reduction, with the highest reduction observed with monk fruit (4.18 log CFU/g) and the lowest with sucralose (3.47 log CFU/g). Notably, B. subtilis strains exhibited significantly greater viability during baking compared to L. acidophilus (p < 0.05). Furthermore, Bacillus probiotics maintained their viability even under high-temperature baking conditions, suggesting their potential for use in baked goods as a viable probiotic ingredient. Additionally, the use of sugar alternatives, such as monk fruit, sucralose, and stevia, was found to significantly increase the water activity in baked cookies, which may contribute to decreased stability and preservation of probiotic efficacy. This study underscores the superior stability of Bacillus probiotics in baked products and highlights the potential benefits of using sugar substitutes to enhance both product shelf life and health benefits.