# Analysis of Genetic Diversity and Core Germplasm Construction of Castanea crenata Siebold and Zucc. Using Simple Sequence Repeat Markers and Morphological Traits

## TL;DR

This study uses genetic and morphological data to analyze the diversity and relationships of Japanese chestnut and related species, constructing a core set of germplasm resources for conservation and use.

## Contribution

A novel core germplasm set for Japanese chestnut is constructed using SSR markers and morphological traits, validated for diversity preservation.

## Key findings

- Japanese chestnut and C. seguinii form a sister clade, showing a close evolutionary relationship.
- 142 Japanese chestnut resources were grouped into two branches with significant genetic admixture.
- 41 core germplasm resources were selected to effectively preserve the genetic diversity of the original set.

## Abstract

This study investigates the taxonomic status, phylogenetic relationships, and genetic diversity of Japanese chestnut (Castanea crenata Siebold & Zucc.) in Liaodong, China, and across East Asia. Additionally, it evaluates core germplasm resources through cluster and population structure analyses using simple sequence repeat (SSR) marker data from 13 Castanea henryi, 18 Castanea seguinii, and 27 Castanea mollissima, and 142 Japanese chestnut resources. The results show that the East Asian Castanea genus forms a monophyletic group with distinct interspecific boundaries. Japanese chestnut and two varieties/lines of C. seguinii (187 and 170) form a sister clade, indicating a close phylogenetic relationship. All Japanese chestnut resources are divided into two branches, with considerable admixture. The genetic diversity analysis revealed that the 142 Japanese chestnut varieties/lines collectively possessed 141 allelic loci, with genetic distances (GDs) ranging from 0.429 to 0.880 with an average of 0.740. Based on unique characteristics, seven resources with distinctive features were selected as mandatory. A total of 41 core germplasm resources were finally determined using the simulated annealing method. The comparative analysis revealed that, aside from a notable difference in polymorphic information loci, the core germplasm and original germplasm showed no significant differences in other genetic diversity parameters. This indicates that the 41 core germplasm resources effectively preserve the genetic diversity of the original germplasm and have been influenced by artificial selection. This study provides a scientific basis for conserving and using C. crenata germplasm resources.