# Estrogenic Effect of Various Plant Extracts on Eel (Anguilla japonica) Hepatocytes

## TL;DR

This study examines how plant extracts affect estrogen-related gene activity in eel liver cells, identifying Schisandra and Astragalus as having strong estrogen-like effects.

## Contribution

The study is the first to investigate phytoestrogen activity in relation to eel maturation and identifies key active compounds in plant extracts.

## Key findings

- Schisandra and Astragalus extracts significantly increased vtg mRNA expression in eel hepatocytes.
- Gomisin N and formononetin were identified as the main phytoestrogens responsible for the observed estrogenic effects.
- Only Schisandra and soybean extracts increased esr mRNA expression in eel hepatocytes.

## Abstract

Estrogen plays some important roles in many physiological processes in animals. This hormone is used as a type of medication for humans and animals, including fish, but is associated with serious side effects and environmental persistence, which has led to a growing interest in phytoestrogens as an alternative. Phytoestrogens are compounds derived from plants that are structurally similar to estrogen and may exhibit similar behavior in the body. To date, no studies have investigated the activity of phytoestrogens in relation to the maturation of eels. In the present study, we investigated the effects of ten different plant extracts on vitellogenin (vtg) and estrogen receptor (esr1, esr2) gene expression in eel hepatocytes. As a result, Schisandra and Astragalus extracts induced higher levels of vtg mRNA expression compared to the other extracts. However, increased esr mRNA expression was observed only in the Schisandra and soybean extract-treated groups. The phytoestrogens known to be present in Schisandra and Astragalus were analyzed using HPLC. Schizandrin, gomisin A, and gomisin N were detected in Schisandra extract, and calycosin and formononetin were detected in Astragalus extract. We then examined whether these phytoestrogens could induce vtg mRNA expression in eel hepatocytes. As a result, gomisin N and formononetin significantly induced vtg mRNA expression. In conclusion, among the 10 plant extracts treated in this study, Schisandra and Astragalus extracts induced estrogenic activity in eel hepatocytes. These extracts were found to contain phytoestrogens, with gomisin N and formononetin identified as the primary active components responsible for the observed estrogenic activity in eel hepatocytes.