# Impact of Corneal Crosslinking on Endothelial and Biomechanical Parameters in Keratoconus

## TL;DR

This study examines how corneal crosslinking affects corneal structure and biomechanics in keratoconus patients before and after treatment.

## Contribution

The study provides new insights into the impact of corneal crosslinking on endothelial and biomechanical parameters across different stages of keratoconus.

## Key findings

- Advanced keratoconus stages show higher endothelial cell variability and lower cell numbers in the cornea.
- Corneal crosslinking leads to a decrease in paracentral endothelial cell count and an increase in cell variability and surface.
- Biomechanical parameters like corneal hysteresis and resistance factor correlate strongly with topography measurements.

## Abstract

Background/Objectives: Keratoconus (KC) is a corneal ectatic disease, characterized by the progressive thinning of the cornea, myopia, and astigmatism, which lead to a decrease in visual acuity. Corneal collagen crosslinking (CXL) is an efficient method of stopping the progression of the disease. The objective of this study is to investigate the endothelial and biomechanical properties of the cornea in keratoconus patients, before and after undergoing corneal collagen crosslinking. Methods: A total of 66 eyes were diagnosed with progressive keratoconus and were recommended epi-off corneal crosslinking. Before the procedure, they were investigated with corneal topography (for minimum, maximum, average keratometry, and corneal astigmatism), specular microscopy (for the following endothelial cell parameters: number, density, surface, variability, and hexagonality), and an ocular response analyzer (for the following biomechanical parameters: corneal hysteresis and resistance factor). All measurements were repeated 1 month and 6 months after the intervention. Results: Several parameters differ according to the Amsler–Krumeich stage of keratoconus: in more advanced stages, patients present higher endothelial cell variability, a lower number of endothelial cells in the paracentral region of the cornea, lower CCT and CRF, and higher keratometry and astigmatism. Endothelial cell variability and number correlate with average keratometry, and there are also strong correlations between topography and CH and CRF. After CXL, the paracentral number of endothelial cells decreased; cell variability and average cell surface increased. Conclusions: More advanced keratoconus cases present with altered corneal biomechanics and topographical parameters, the endothelial layer also being affected proportional to the stage of the disease and also slightly affected after corneal collagen crosslinking.