# Characterizing zonulin and par2 Expression in Zonulin Transgenic and Zonulin Inhibition Mouse Models of Motility and Inflammation

## TL;DR

This study explores how zonulin and its receptor PAR2 affect gut motility and inflammation in mice, finding that PAR2 plays a key role in zonulin's effects during inflammation.

## Contribution

The study reveals that PAR2 is critical for zonulin's impact on motility during inflammation, despite zonulin being expressed in all models.

## Key findings

- Zonulin transgenic mice showed reduced GI transit slowing after LPS compared to wildtype mice.
- Zonulin inhibition restored motility patterns to those of wildtype mice during inflammation.
- Zonulin expression was upregulated in all models after LPS, but PAR2 appears essential for its motility effects.

## Abstract

We aimed to examine the effect of zonulin and zonulin inhibition on gastrointestinal (GI) motility and the mRNA expression of zonulin and the protease-activated receptor 2 (par2), the primary receptor for zonulin, under conditions of inflammation by lipopolysaccharide (LPS) injection. The experimental models included zonulin transgenic mice (ztm), par2 knockout ztm (ztm-par2 −/−), ztm exposed to the zonulin inhibitor AT1001 (ztm-AT1001), and wildtype mouse controls. GI transit was measured by fluorescein isothiocyanate-dextran and mRNA expression by real-time quantitative polymerase chain reaction in whole, and in epithelial and non-epithelial tissues of all GI segments. There were no differences in the GI transit between mouse groups at baseline. After the LPS injection, ztm mice had an attenuated slowing of the GI transit compared to wildtype mice. The zonulin-inhibited mice had motility patterns similar to wildtype mice. zonulin upregulation was noted in GI segments of the ztm, ztm-par2 −/−, and ztm-AT1001 after the LPS injection. Differences in motility patterns between ztm and zonulin inhibition models despite zonulin expression in GI segments of all mouse groups supports that PAR2 is key for zonulin’s effect on motility under conditions of inflammation. However, the findings from the epithelial and non-epithelial compartments suggest that the pathway of activity is complex and likely indirect.