# Role of LncRNA MRPS28 in Secondary Hair Follicle Development of Cashmere Goats

## TL;DR

This study identifies lncRNA MRPS28 as a key regulator of secondary hair follicle development in cashmere goats, affecting cashmere quality and yield.

## Contribution

The study is the first to elucidate the molecular mechanism of lncRNA MRPS28 in regulating secondary hair follicle development via the chi-miR-145-5p/NUAK1 axis.

## Key findings

- lncRNA MRPS28 inhibits dermal fibroblast proliferation and migration.
- lncRNA MRPS28 promotes dermal fibroblast apoptosis.
- lncRNA MRPS28 sponges chi-miR-145-5p to regulate NUAK1, affecting dermal papilla formation.

## Abstract

The development of secondary hair follicles in cashmere goats directly influences the yield and quality of cashmere, and hair follicle morphogenesis is a complex biological process involving multiple signaling pathways and regulatory factors. Through specific screening and analysis, this study identified lncRNA MRPS28, which exhibits significant differential expression during the morphogenesis of secondary hair follicles. The results indicate that lncRNA MRPS28 can inhibit the proliferation and migration of dermal fibroblasts via the chi-miR-145-5p/NUAK1 axis, thereby impeding the formation of dermal papilla structure and ultimately affecting the morphogenesis of secondary hair follicles during the embryonic stage. This study is the first to elucidate the molecular mechanism by which lncRNA MRPS28 regulates the development of secondary hair follicles in cashmere goats via the chi-miR-145-5p/NUAK1 axis. It offers a novel perspective on dissecting the regulatory network of hair follicle development in cashmere goats and provides potential theoretical insights into breeding new strains of cashmere goats.

The development of secondary hair follicles in cashmere goats directly influences the yield and quality of cashmere, and hair follicle morphogenesis is a complex biological process involving multiple signaling pathways and regulatory factors. In recent years, the regulatory role of long non-coding RNAs in hair follicle development has gradually been uncovered. Based on a previously established transcriptome database of embryonic skin tissue from cashmere goats, this study ultimately identified lncRNA MRPS28, which exhibits significant differential expression during the morphogenesis of secondary hair follicles. Our results indicate that lncRNA MRPS28 is a non-coding RNA transcribed from the intron region of the MRPS28 gene. Additionally, it can inhibit the proliferation and migration of dermal fibroblasts while promoting their apoptosis. Further mechanistic studies have shown that lncRNA MRPS28 acts as a competing endogenous RNA, sponging chi-miR-145-5p, thereby relieving its inhibitory effect on the target gene NUAK1. This, in turn, inhibits the formation of dermal papilla structures and ultimately affects the morphogenesis of secondary hair follicles during the embryonic period. This study provides a novel perspective on dissecting the regulatory network of hair follicle development in cashmere goats and offers potential theoretical insights for breeding new strains of cashmere goats.