# Complete sequence of the avian pathogenic Escherichia coli strain VTB96933v containing a novel conjugative antimicrobial resistance and virulence IncI1-ST114-ColBM plasmid

**Authors:** Zhuohao Wang, David Gerber, Vincent Perreten

PMC · DOI: 10.1128/mra.00284-25 · 2025-06-12

## TL;DR

This paper reports the full genetic sequence of a South African bird-disease-causing E. coli strain with a new plasmid carrying drug resistance and virulence genes.

## Contribution

The discovery of a novel conjugative IncI1-ST114-ColBM plasmid with resistance and virulence genes in avian pathogenic E. coli.

## Key findings

- The strain has a 5.12 Mb chromosome and two plasmids, including a 161,786 bp ColBM plasmid.
- The plasmid is a new IncI1-ST114 type carrying antimicrobial resistance and APEC virulence genes.
- The sequence was assembled using short and long read consensus-based methods.

## Abstract

The complete sequence of a South African avian pathogenic Escherichia coli was obtained through short and long read consensus-based assembly. It consists of a 5,120,371 bp chromosome and two plasmids, one of 86,932 and the other of 161,786 bp. The latter is a novel conjugative ColBM plasmid type IncI1-ST114, containing antimicrobial resistance and APEC-associated virulence genes.

## Linked entities

- **Species:** Escherichia coli (taxon 562)

## Full-text entities

- **Species:** Stenotrophomonas sp. T1-14 (species) [taxon 671765], Escherichia coli (E. coli, species) [taxon 562]
- **Cell lines:** VTB96933v — Mus musculus (Mouse), Spontaneously immortalized cell line (CVCL_B9UX)

## Figures

1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC12243562/full.md

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Source: https://tomesphere.com/paper/PMC12243562