# Complete genome sequences of Escherichia coli phages Huey, Dewey, and Louie

**Authors:** Enea Maffei, Luc Willi, Alexander Harms

PMC · DOI: 10.1128/mra.00270-25 · 2025-06-18

## TL;DR

This paper presents the full genome sequences of three new bacteriophages that infect Escherichia coli by targeting a specific cell surface component.

## Contribution

The study provides novel genome sequences of three phages and identifies their receptor specificity and taxonomic classification.

## Key findings

- The genomes of phages Huey, Dewey, and Louie are 38,575, 40,352, and 38,040 bp in size respectively.
- The phages belong to the Lederbergvirus genus and target the O16-type O-antigen of E. coli K-12.
- They are similar to the model phage P22, which infects Salmonella Typhimurium.

## Abstract

Bacteriophages Huey, Dewey, and Louie are new viruses infecting Escherichia coli K-12 that target its O16-type O-antigen as the host receptor. We report the genomes of these phages, which are 38,575, 40,352, and 38,040 bp in size. These viruses belong to the Lederbergvirus genus of podoviruses, similar to the well-studied model phage P22, which infects Salmonella Typhimurium.

## Linked entities

- **Species:** Escherichia coli (taxon 562)

## Full-text entities

- **Species:** Lederbergvirus (genus) [taxon 186794], Salmonella (genus) [taxon 590], Escherichia coli (E. coli, species) [taxon 562]

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Source: https://tomesphere.com/paper/PMC12243541