# Unveiling the genomic architecture of multidrug-resistant Pseudomonas sp. RTCS2 isolated from spoiled Solanum lycopersicum L

**Authors:** Shubhajit Shaw, Rittick Mondal, Pankaj Mandal, Avijit Mandal, Ritwik Acharya, Dipanjan Das, Sujan Ch. Paul, Arka Pratim Chakraborty, Debnirmalya Gangopadhyay, Amit Kumar Mandal

PMC · DOI: 10.1128/mra.00337-25 · 2025-06-09

## TL;DR

This paper presents the draft genome of a Pseudomonas sp. RTCS2 bacterium isolated from spoiled tomatoes in India, revealing its potential to resist antimicrobials.

## Contribution

The study provides the first genomic characterization of Pseudomonas sp. RTCS2, identifying antimicrobial resistance genes.

## Key findings

- The genome of Pseudomonas sp. RTCS2 is 5,526,005 bp with a 62.9% G + C content.
- Antimicrobial resistance genes adeF, vanH, and vanT were identified in the genome.
- The bacterium shows pathogenic potential due to the presence of resistance genes.

## Abstract

Here, we report the draft genome of Pseudomonas sp. RTCS2, a gram-negative rod-shaped bacterium associated with tomato spoilage, isolated from Raiganj University, India (25.6071° N; 88.1306° E). The 5,526,005 bp genome with a 62.9% G + C content harbors antimicrobial resistance genes (adeF, vanH, and vanT), highlighting its pathogenic potential.

## Linked entities

- **Genes:** adeF (multidrug efflux RND transporter periplasmic adaptor subunit AdeF) [NCBI Gene 9381527], vanH (VanH-AOV family D-lactate dehydrogenase) [NCBI Gene 29590529], vanT (serine racemase VanT catalytic subunit) [NCBI Gene 42857389]
- **Species:** Pseudomonas sp. RTCS2 (taxon 3389877)

## Full-text entities

- **Species:** Pseudomonas sp. (species) [taxon 306], Solanum lycopersicum (tomato, species) [taxon 4081]

## Figures

1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC12243486/full.md

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Source: https://tomesphere.com/paper/PMC12243486