# Characterization of prokaryotic communities in Puerto Rican caves using 16S rDNA amplicon sequencing

**Authors:** Natalia Pérez-Santos, Sebastian Javier Borrero-Villabol, Rene Nieves-Morales, Jessica Alejandra Paez-Díaz, Edwin Omar Rivera-Lopez, Josué Rodríguez-Ramos, Angel M. Nieves-Rivera, Carlos Ríos-Velazquez

PMC · DOI: 10.1128/mra.00354-25 · 2025-06-10

## TL;DR

This study explores the unique prokaryotic communities in Puerto Rican caves using DNA sequencing to better understand subterranean ecosystems.

## Contribution

The study provides new insights into prokaryotic diversity in Puerto Rican caves using 16S rDNA amplicon sequencing.

## Key findings

- Distinct prokaryotic communities were identified across seven Puerto Rican caves.
- The findings enhance understanding of microbial adaptation in extreme subterranean environments.

## Abstract

The cave ecosystems host microbial communities adapted to extreme environments. This study utilized 16S rDNA to investigate the prokaryotic diversity across seven caves in Puerto Rico’s northern limestone karst belt. Microbial profiling revealed distinct subterranean communities, enhancing our understanding of cave microbiology and potential applications in environmental conservation and microbial research.

## Full-text entities

- **Genes:** CAT (catalase) [NCBI Gene 847]
- **Chemicals:** SDS (MESH:D012967), GITC (MESH:C041943), nitrogen (MESH:D009584), Guanidine Isothiocyanate (MESH:C054435), manganese (MESH:D008345), antiviral compounds (-), iron (MESH:D007501), nitrates (MESH:D009566)
- **Species:** Bacillus sp. AT (species) [taxon 1196779], Pseudomonadota (proteobacteria, phylum) [taxon 1224]

## Figures

1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC12243483/full.md

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Source: https://tomesphere.com/paper/PMC12243483