Intestinal microbiome profile of the brown rock sea cucumber (Holothuria glaberrima) using ITS and 16S rDNA amplicons from direct mechanical, enzymatic, and chemical metagenomic extraction

TL;DR

This study explores the gut microbiome of a sea cucumber using different DNA extraction methods and sequencing techniques.

Contribution

The paper introduces a comparative analysis of microbiome extraction methods in a marine invertebrate.

Findings

Different extraction methods yielded varying profiles of prokaryotic and fungal diversity.

ITS and 16S rDNA sequencing revealed distinct microbial communities across intestinal regions.

Abstract

Using direct mechanical, enzymatic, and chemical extraction methods, the intestinal microbiome of the marine invertebrate Holothuria glaberrima was obtained. ITS and 16S rDNA regions were sequenced to enrich and investigate the prokaryotic and fungal diversity profiles from different anatomical regions within the sea cucumber’s intestinal biology.

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