Evaluation of filaggrin 2 expression in dogs with atopic dermatitis before and after oclacitinib maleate administration

TL;DR

This study found that oclacitinib maleate improved skin barrier function in dogs with atopic dermatitis by increasing filaggrin 2 expression.

Contribution

The study demonstrates that oclacitinib maleate can enhance FLG2 expression in atopic dogs, suggesting a role in improving skin barrier function.

Findings

Control dogs showed higher FLG2 expression than atopic dogs on Day 0.

FLG2 expression in atopic nonlesional skin increased significantly after oclacitinib treatment.

No significant difference in FLG2 expression was found between control and treated atopic nonlesional skin on Day 30.

Abstract

Canine atopic dermatitis (cAD) is a chronic, inflammatory, multifactorial and pruritic disease. The presence of skin barrier impairment (e.g. filaggrin alterations), along with abnormal immune responses, can negatively impact cutaneous barrier function.

To evaluate the filaggrin 2 (FLG2) expression in atopic dogs before and after the administration of oclacitinib maleate.

Sixteen privately owned dogs with a diagnosis of cAD and 10 healthy control dogs.

Oclacitinib maleate monotherapy at 0.5 mg/kg, orally, twice‐daily for the first 14 days and once‐daily for 16 additional days, was administered to the atopic dogs. Skin biopsies from lesional and nonlesional skin were obtained from atopic dogs on Day(D)0 and D30 and from the same anatomical locations from the control group on D0. Immunohistochemical investigation was performed using a primary custom‐made anti‐canine‐filaggrin 2 polyclonal antibody. Immunolabelled slides were scanned and FLG2 expression was measured. Data were analysed and a p‐value ≤0.05 was considered statistically significant.

There was a higher FLG2 expression in control skin when compared with atopic skin (lesional and nonlesional) on D0 (p = 0.033). FLG2 expression comparison between control and D30 (nonlesional) did not show a significant difference (p = 0.509). A significant increase in FLG2 expression in atopic nonlesional skin on D30 compared with nonlesional skin on D0 was also observed (p = 0.014).

Oclacitinib maleate could have a positive impact on cutaneous barrier structure, improving FLG2 expression by decreasing inflammation and cutaneous trauma.

Background – Canine atopic dermatitis (cAD) is a chronic, inflammatory, multifactorial and pruritic disease. The presence of skin barrier impairment (e.g. filaggrin alterations), along with abnormal immune responses, can negatively impact cutaneous barrier function. Objectives – To evaluate the filaggrin 2 (FLG2) expression in atopic dogs before and after the administration of oclacitinib maleate. Conclusions and Clinical Relevance – Oclacitinib maleate could have a positive impact on cutaneous barrier structure, improving FLG2 expression by decreasing inflammation and cutaneous trauma.

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