# Synthetic feed-forward loop circuit boosts transgene expression in sugarcane

**Authors:** Zhihai Zhang, Sultana Anwar, Erin J. Yafuso, Evelyn Tatiana Zuniga Soto, Changwei Li, Guangbin Luo, Stephen P. Moose, Kankshita Swaminathan, Fredy Altpeter, Matthew E. Hudson

PMC · DOI: 10.1007/s00299-025-03561-3 · Plant Cell Reports · 2025-07-08

## TL;DR

A new genetic circuit boosts gene expression in sugarcane plants, improving the performance of a reporter gene in leaves and stems.

## Contribution

A GAL4-based feed-forward loop circuit is developed to enhance transgene expression in sugarcane.

## Key findings

- The GAL4-based circuit increases β-glucuronidase (GUS) reporter gene expression in sugarcane.
- Enhanced expression is observed in both leaves and stems of transformed plants.
- The circuit is effective in stably transformed sugarcane plants.

## Abstract

A new GAL4-based feed-forward loop circuit enhances β-glucuronidase (GUS) reporter gene expression in leaves and stems of stably transformed sugarcane plants.

## Linked entities

- **Genes:** LGALS4 (galectin 4) [NCBI Gene 3960], gus (gustavus) [NCBI Gene 35478]

## Full-text entities

- **Chemicals:** BS (MESH:D001895), 4-MU (MESH:D006923), geneticin (MESH:C010680), DE (MESH:D004054), 4-MUG (-), oil (MESH:D009821)
- **Species:** Sorghum bicolor (broomcorn, species) [taxon 4558], Arabidopsis thaliana (mouse-ear cress, species) [taxon 3702]
- **Cell lines:** pBEC64-10 — Homo sapiens (Human), Acute myeloid leukemia, Cancer cell line (CVCL_8074), pBEC64-4 — Mus musculus (Mouse), Hybridoma (CVCL_B7CZ)

## Full text

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## Figures

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Source: https://tomesphere.com/paper/PMC12238179