# SUPPRESOR OF GAMMA RESPONSE 1 promotes early onset of endoreplication upon DNA double-strand breaks by inducing CCS52A1 expression in Arabidopsis roots

## TL;DR

This study shows how the SOG1 protein helps plants start endoreplication quickly after DNA damage by activating CCS52A1 in Arabidopsis roots.

## Contribution

The study reveals that SOG1 induces CCS52A1 to promote early endoreplication in response to DNA damage in plants.

## Key findings

- SOG1 directly binds to and activates the CCS52A1 gene after DNA double-strand breaks.
- CCS52A1 is essential for the early onset of endoreplication in Arabidopsis roots.
- Increased polyploidy may be an adaptive response to DNA damage in plants.

## Abstract

Living organisms are constantly at the risk of DNA damage caused by factors such as DNA replication errors, reactive oxygen species, and UV radiation. In plants, DNA damage activates the NAC-type transcription factor SUPPRESSOR OF GAMMA RESPONSE 1 (SOG1) that governs DNA damage responses such as cell cycle arrest, stem cell death, and early onset of endoreplication. However, molecular mechanisms underlying the early induction of endoreplication onset in response to DNA damage remain elusive. In this study, we show that CELL CYCLE SWITCH 52 A1 (CCS52A1), an activator of the APC/C E3 ligase, plays a major role in the early onset of endoreplication in response to DNA double-strand breaks (DSBs) in Arabidopsis roots. Upon DSBs, SOG1 directly binds to the CCS52A1 locus, thereby inducing its expression. Moreover, early transition to endoreplication in response to DSBs is suppressed in ccs52a1 knockout roots. Our data propose that increased polyploidy may function as a unique adaptative mechanism to genotoxic stress in plants.

The online version contains supplementary material available at 10.1007/s10265-025-01630-y.