# Effect of Andrographis paniculata and Melia azadirachta against the Dengue virus

## TL;DR

This study shows that two plants, Andrographis paniculata and Melia azadirachta, have strong antiviral effects against Dengue virus-1 without harming cells.

## Contribution

The study demonstrates the antiviral potential of A. paniculata and M. azadirachta against Dengue virus-1 using in vitro assays.

## Key findings

- Both plant extracts showed potent antiviral activity at 150 µg/ml against Dengue virus-1.
- The extracts were non-toxic up to 1.5 mg/ml, with 100% cell viability observed.
- GC-MS analysis identified phytochemicals in the plant extracts.

## Abstract

Dengue infection is emerging as a global threat due to increased mortality and morbidity, especially in tropical and sub-tropical
regions. Therefore, it is of interest to evaluate the antiviral activity of Andrographis paniculata and Melia
azadirachta against Dengue virus-1 in a vero cell line using a cytopathic effects (CPE) reduction assay and
cytotoxicity assessed by MTT assay. GC-MS analysis was conducted to identify phytochemicals in the plant extracts. Both extracts showed
potent antiviral activity at 150 µg/ml and were non-toxic up to 1.5 mg/ml, with 100% cell viability. These findings suggest that
A. paniculata and M. azadirachta are promising candidates against Dengue virus-1,
warranting further investigation into their active compounds.