# Molecular profile PCR array of regenerative therapy (PRP, PRF& CpG-ODN) in wound healing of diabetic dogs

## TL;DR

This study examines how regenerative therapies like PRP, PRF, and CpG-ODN affect wound healing in diabetic dogs by analyzing gene expression and tissue changes.

## Contribution

The study introduces a novel application of RT2 Profiler PCR array to evaluate gene expression in diabetic wound healing using regenerative therapies in dogs.

## Key findings

- PRP combined with CpG-ODN showed the highest wound healing rate and collagen production in diabetic dogs.
- The treatment upregulated 37 genes and downregulated 10 genes related to wound healing.
- PRP & CpG-ODN outperformed other treatments and the control group in wound contraction and epithelialization.

## Abstract

This study’s aim is to validate differential wound healing genes expression in canine with diabetes using regenerative medicine such as PRP, PRF, and CpG oligodeoxynucleotides (ODN), for these purpose thirty six diabetic dogs were used and subdivided into 6 groups (6 dogs each) with full-thickness incisional wounds, wound healing was evaluated with a focus on histological changes in wound tissue and gene expression after 3 weeks. The molecular mechanism of wound healing responding to different treatments in dogs were done using the new molecular technique RT2 Profiler PCR array (Qiagen), which enables us to view a focused panel of genes (84 genes) responsible for wound healing. CpG-ODN contributes to low cellular infiltration; PRP improves diabetic wound closure rapidly. The study illustrated that the healing rate was higher in the PRP & CpG-ODN. The animal in this group showed a clinically higher contraction rate of the wound area, upregulates 37 genes responsible for wound healing and downregulates ten genes for the same purpose, also marked collagen production were observed with complete epithelialization. Our study concluded that PRP & CpG-ODN was superior to PRF & CpG-ODN, PRP, PRF, CpG-ODN, and the control group, respectively, for enhancing wound healing in diabetic dogs.

The online version contains supplementary material available at 10.1186/s12917-025-04892-9.