Interaction of PsMYB4 with PsEGL3 inhibits anthocyanin biosynthesis in tree peony yellow flowers
Xiaoning Luo, Sijie Huang, Fangjun Jiang, Qianqian Shi, Yafeng Wen, Mengchen Li, Minhuan Zhang, Yanlong Zhang
TL;DR
This study identifies two genes, PsMYB4 and PsEGL3, that work together to block anthocyanin production in yellow tree peony flowers, explaining their yellow coloration.
Contribution
The novel contribution is the discovery that PsMYB4 and PsEGL3 interact to suppress anthocyanin biosynthesis in yellow tree peony flowers.
Findings
PsMYB4 and PsEGL3 are transcription factors that inhibit anthocyanin synthesis in yellow tree peony flowers.
PsMYB4 overexpression strongly reduces anthocyanin-related gene expression in tobacco.
PsMYB4 and PsEGL3 together suppress the promoters of key anthocyanin biosynthesis genes.
Abstract
Tree peony is a traditional woody flower originating from China and possesses high ornamental value. The yellow cultivar is even more precious, known as the highest grade. However, the molecular mechanism underlying tree peony yellow flower formation is still unclear. Our present work identified two transcription factors (TFs), PsMYB4 and PsEGL3, which were highly expressed in yellow tree peony cultivar. Phylogenetic analysis indicates that PsMYB4 belonged to the R2R3-MYB repressor, while PsEGL3 was clustered into subgroup IIIf of bHLH family. Overexpression of PsMYB4 and PsEGL3 respectively in tobacco inhibited anthocyanin synthesis, with PsMYB4 overexpressing lines being more pronounced and the expression levels of structural genes NtC4H, NtCHS, NtCHI, and NtDFR were significantly downregulated, while the expression levels of structural genes in PsEGL3 transgenic lines showed no…
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Taxonomy
TopicsPlant Gene Expression Analysis · Phytochemicals and Antioxidant Activities · Plant biochemistry and biosynthesis