PLGA nanoparticles as an efficient carrier in Toxoplasma GAP45: a more effective vaccine against acute toxoplasmosis than traditional ones
Pan Zhou, YanLi Yu, WeiYu Qi, XiaoJuan Wang, YouLi Yu, JianDong Wang, Li Zhang, ZhengQing Yu, TingLi Liu
TL;DR
This study shows that a new vaccine using PLGA nanoparticles and TgGAP45 protein offers better protection against toxoplasmosis than traditional vaccines.
Contribution
The use of PLGA nanoparticles as a delivery system for TgGAP45 is novel and demonstrates superior immunoprotection against T. gondii.
Findings
TgGAP45-PLGA nanospheres induced a mixed Th1/Th2 immune response.
TgGAP45-PLGA nanospheres provided the strongest immunoprotection in spleen and heart tissues.
The vaccine formulation outperformed traditional oil adjuvant-based vaccines.
Abstract
Toxoplasma gondii (T. gondii), as a strict intracellular parasite, can infect nearly all mammals, including humans, posing significant threats to public health. Toxoplasmosis in animals also leads to substantial economic losses in animal husbandry. Currently, no effective treatments are available for toxoplasmosis, creating an urgent need for safe and efficient therapeutics. In this study, we constructed a subunit vaccine using T. gondii glidesome-associated protein 45 (TgGAP45). To enhance immunogenicity, poly (lactic-co-glycolic acid) (PLGA) nanoparticles were employed as delivery carriers to prepare TgGAP45-PLGA nanospheres. For comparison, two oil adjuvants, Montanide™ ISA 660 VG and Montanide™ ISA 206 VG, were used to formulate TgGAP45-206VG and TgGAP45-660VG emulsions. Following safety evaluation, protective immunity was assessed in animals. Antibody levels, cytokine profiles,…
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Taxonomy
TopicsToxoplasma gondii Research Studies · Parasitic Infections and Diagnostics · Herpesvirus Infections and Treatments