# Accounting for shear scission in the size-exclusion chromatography of debranched starch

## TL;DR

This paper introduces a method to correct for shear scission in size-exclusion chromatography when analyzing debranched starch, improving the accuracy of chain-length distribution measurements.

## Contribution

A novel method to correct shear scission effects in SEC by extrapolating model-based parameters to zero flow rate.

## Key findings

- Shear scission significantly degrades extra-long amylose chains during SEC.
- The correction method improves the reliability of starch structural and biosynthesis inferences.
- Extrapolation to zero flow rate reveals more accurate chain-length distributions.

## Abstract

The chain-length distribution (CLD) of starch influences many functional properties of starch-containing substances, and also contains information about starch biosynthetic processes. The commonest method for measuring this CLD is to debranch the starch enzymatically, and then to measure the molecular weight distribution of the resulting linear chains using size-exclusion chromatography (SEC). However, SEC suffers from various artifacts, including shear scission of longer chains. Here, a method of correcting for such shear scission is developed: fitting the apparent CLDs (affected by shear scission) with biosynthesis-based models, over a range of flow rates, and extrapolating the resulting model-based parameters to zero flow rate. To apply this, the apparent CLDs of five rice starches were measured using SEC with a range of flow rates, and these apparent CLDs were parameterized using biosynthesis-based models. The model parameters fitted from CLDs at different flow rates were extrapolated to zero flow rate, and were then used to calculate the CLD that would be obtained with zero flow rate, thereby taking partial account of shear scission. The extrapolation suggests that shear scission significantly degrades extra-long (degree of polymerization, DP, >1500) amylose chains to shorter chains (DP ~ 500–1500), which, if uncorrected, would vitiate inferences from SEC results. The partial correction method devised here can be used to develop more reliable relationships between structural and biosynthesis-related parameters of starch and functional properties of starch-containing substances.