# MINFLUX: μs and nm precision 3D tracking of dynamic lipid mobility on nanoparticles

## TL;DR

MINFLUX microscopy enables precise 3D tracking of lipid dynamics on nanoparticles with microsecond and nanometer resolution.

## Contribution

A framework for MINFLUX imaging and data analysis tailored to nanosized Silica-Supported Lipid Bilayers (SSLBs) is introduced.

## Key findings

- MINFLUX achieves μs temporal and nm spatial resolution in 3D.
- The method is applied to study dynamic lipid mobility on SSLBs.
- The framework aids in understanding complex nanomaterial dynamics.

## Abstract

MINFLUX microscopy opened unique opportunities to investigate complex molecular systems at a single molecule level with μs temporal and nm spatial resolutions in 3D. Here, we demonstrate its use for the characterization of nanomaterials, providing a framework for MINFLUX imaging and data analysis tailored to understanding the complex dynamics of nanosized Silica-Supported Lipid Bilayers (SSLBs).

MINFLUX microscopy opened unique opportunities to investigate complex molecular systems at a single molecule level with μs temporal and nm spatial resolutions in 3D.