# Persistent lymphopenia after kidney transplantation: increased mortality and decreased homeostatic mechanisms

## TL;DR

This study finds that many kidney transplant patients have long-term low lymphocyte counts, which is linked to higher mortality and suggests problems with immune cell homeostasis.

## Contribution

The study is the first to detail persistent lymphopenia after kidney transplantation and its association with mortality and immune homeostasis.

## Key findings

- Persistent lymphopenia affects 31% of kidney transplant recipients three years post-transplant.
- Lower lymphocyte counts correlate with increased mortality risk.
- Persistent lymphopenia is linked to impaired T, B, and NK cell homeostasis and increased PD-1 expression.

## Abstract

Kidney transplant (KTx) recipients commonly develop transient lymphopenia due to treatment with alemtuzumab, rabbit anti-thymocyte globulin (rATG) or other conditions. However, persistent lymphopenia lasting for years has not been studied in detail. The goal of this study was to determine the prevalence of persistent lymphopenia, evaluate its association with mortality and investigate possible mechanisms by which it occurs.

We retrospectively studied peripheral blood lymphocyte and leukocyte counts in 7307 adult, solitary renal transplant recipients transplanted between 1/2006 to 12/2020.

While leukocyte counts generally remained within the normal range, lymphocyte counts 3 years after KTx were below normal in 31% (compared to 14% pretransplant and 54% at 1 year). Increasing severity of lymphopenia at 3 years was associated with increasing risk of mortality. The major risk factors for lymphopenia at 3 years were: receiving alemtuzumab or rATG for induction or the treatment of rejection, increasing recipient age, pretransplant dialysis, a low lymphocyte count pretransplant, and having a prior kidney transplant. Mass cytometry immunophenotyping at 3 years showed that B cells, NK cells and all T cell subsets (CD4, CD8, naïve, memory, etc.) decreased with decreasing lymphocyte counts. This included fewer recent thymic emigrants, naïve T cells, and stem-cell like memory T cells (TSCM), suggesting an impaired homeostasis of peripheral T cells. PD-1 expression was increased with decreasing lymphocyte counts in T and B cells and in most T cell subsets including CD4 TSCM, CD4 and CD8 naïve cells, and CD4 recent thymic emigrants.

We conclude that persistent lymphopenia might be partially due to impaired homeostatic mechanisms in T, B and NK cells and might be a simple, useful biomarker for individualizing patient management.