Whole genome sequencing and functional annotation of Fusarium oxysporum f. sp. lentis to unravel virulence and secondary metabolite biosynthesis gene clusters
Sanjay Kharte, Ashish Kumar, Priyamvada Mishra, R. S. Ramakrishnan, Stuti Sharma, Nishi Mishra, Puneet Singh Chauhan, Radheshyam Sharma, Vedant Gautam, Shweta Tiwari, Vinod Goyal, Sonu Sharma, G. K. Koutu, N. K. Joshi
TL;DR
This study sequenced the genome of a lentil crop pathogen to understand its virulence and secondary metabolite production, providing insights for disease management.
Contribution
The study provides the first comprehensive genome assembly and functional annotation of Fusarium oxysporum f. sp. lentis.
Findings
The genome assembly is 99% complete with 116,998 protein-coding genes.
16,779 carbohydrate-active enzymes suggest potential for plant cell wall degradation.
77 biosynthetic gene clusters were identified, including those for polyketide and peptide synthetases.
Abstract
Fusarium oxysporum f. sp. lentis is a major fungal pathogen that causes vascular wilt in lentil crops, leading to significant reductions in yield. Despite its importance, the genetic underpinnings of this pathogen remain poorly understood. We performed whole-genome sequencing of F. oxysporum f. sp. lentis using the Illumina Shotgun Sequencing platform. The resulting high-quality genome assembly consisted of 12,366 contigs with a total length of 124.48 Mb. Genome completeness was evaluated using Benchmarking Universal Single-Copy Orthologs (BUSCO) analysis, and functional annotation was performed through comparisons with several public databases, including Uniprot, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Pfam, and Clusters of Orthologous Groups (COG). Pathogenicity-related genes were identified using the PHI-base database, and secondary metabolite…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
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Taxonomy
TopicsPlant Pathogens and Fungal Diseases · Plant-Microbe Interactions and Immunity · Mycorrhizal Fungi and Plant Interactions