# NGPINT V3: a containerized orchestration Python software for discovery of next-generation protein–protein interactions

## TL;DR

NGPINT V3 is an updated software for analyzing protein–protein interactions using containerization to improve compatibility and ease of use.

## Contribution

NGPINT V3 introduces containerized deployment with Singularity and Docker for cross-platform accessibility and streamlined workflows.

## Key findings

- Container images are hosted on Sylabs and Dockerhub for easy integration into high-throughput and cloud workflows.
- The software is now more compatible and easier to install, resolving previous dependency issues.
- Full documentation and software are available on GitHub and Zenodo.

## Abstract

Batch yeast two-hybrid (Y2H) assays, leveraged with next-generation sequencing, have afforded successful innovations for the analysis of protein–protein interactions. NGPINT is a Conda-based software designed to process the millions of raw sequencing reads resulting from Y2H–next-generation interaction screens. Over time, increasing compatibility and dependency issues have prevented clean NGPINT installation and operation. A system-wide update was essential to continue effective use with its companion software, Y2H-SCORES. We present NGPINT V3, a containerized implementation built with both Singularity and Docker, allowing accessibility across virtually any operating system and computing environment.

This update includes streamlined dependencies and container images hosted on Sylabs (https://cloud.sylabs.io/library/schuyler/ngpint/ngpint) and Dockerhub (https://hub.docker.com/r/schuylerds/ngpint), facilitating easier adoption and integration into high-throughput and cloud-computing workflows. Full instructions and software can be also found in the GitHub repository https://github.com/Wiselab2/NGPINT_V3 and Zenodo https://doi.org/10.5281/zenodo.15256036.