# Immediate postoperative effects of cytoreductive surgery with hyperthermic intraperitoneal chemotherapy using carboplatin on peritoneal tissue inflammatory and ischemic responses: an explorative porcine study

## TL;DR

This study examines the immediate effects of a cancer treatment on pig peritoneal tissue, finding a quick inflammatory response and stable ischemic markers.

## Contribution

The study provides new insights into the short-term inflammatory and ischemic tissue responses following CRS with HIPEC in a porcine model.

## Key findings

- An immediate increase in 27 inflammatory proteins was observed within 30 minutes after HIPEC initiation.
- Hyperlactatemia was constant during and after HIPEC, but no significant tissue ischemia was detected.
- Glucose levels remained stable, while glycerol concentrations tended to decrease over time.

## Abstract

This explorative porcine study investigated the immediate postoperative response of inflammatory protein markers and ischemic metabolites in peritoneal abdominal wall tissue during and after hyperthermic intraperitoneal chemotherapy (HIPEC) assessed by microdialysis.

Eight cancer-free female pigs underwent imitation cytoreductive surgery (CRS) followed by HIPEC (90 min) using a carboplatin dosage of 800 mg/m2. A subperitoneal microdialysis catheter was placed in the abdominal wall to sample inflammatory protein markers and ischemic metabolites. During and after HIPEC, dialysates and blood samples were collected over 8 h. Inflammatory protein expression levels were quantified using Proximity Extension Assay (Olink Target 96 Inflammation panel). In addition, concentrations of ischemic metabolites were quantified using a CMA600 microdialysis analyzer.

An immediate response of 27 proteins, including proteins from the TNF family, early inflammatory chemokines, pro- and anti-inflammatory proteins, was found within the first 30 min after HIPEC initiation. This was followed by a modest and relatively constant inflammatory response. Glycerol concentrations tended to decrease during the sampling period, while glucose showed more stable levels. Constant hyperlactatemia exceeding the plasma levels was seen during and after HIPEC, with no significant tissue ischemia as indicated by the lactate/pyruvate ratios.

In a porcine model, CRS with HIPEC leads to an immediate and relatively constant increased inflammatory and ischemic peritoneal tissue short-time response. The explorative findings contribute to the debate on the value of HIPEC in combination with CRS and call for future studies to further investigate the inflammatory and metabolic tissue responses.