# Novel reporter systems to detect cold and osmotic stress responses

## TL;DR

The paper introduces new reporter systems to study how cells respond to cold and osmotic stress.

## Contribution

The study presents novel reporter systems based on intracellular events triggered by cold and osmotic stress.

## Key findings

- A new reporter system was developed to detect intracellular events in response to cold and osmotic stress.
- These systems can help elucidate the signaling mechanisms activated by environmental stress in higher eukaryotes.
- The systems build upon a previously described inducible translocation trap involving KBL.

## Abstract

Cells respond to environmental stresses such as cold and osmotic stresses. These stresses induce signal transduction pathways in cells. However, the molecular mechanisms activated by cold and osmotic stresses in higher eukaryotes remain elusive. Previously, we described a reporter system utilizing inducible translocation trap that detects nuclear translocation of 2-amino-3-ketobutyrate coenzyme A ligase (KBL) in response to cold and osmotic stresses. In the present study, we developed additional reporter systems to detect intracellular events induced by these stresses. These reporter systems will be instrumental to elucidate the intracellular signaling mechanisms activated by these stresses.