PorelisTM, a standardized purple tea extract, improves adipocyte functionality by regulating the leptin and adiponectin levels in high-fat diet-induced C57BL/6 mice

TL;DR

Purple tea extract Porelis reduces weight and fat in mice by regulating leptin and adiponectin levels and inhibiting fat formation.

Contribution

Demonstrates Porelis' antiobesity effects via regulation of adipogenesis and energy homeostasis in HFD-induced mice.

Findings

Porelis reduced body weight, fat pad weights, hepatic lipid accumulation, and oxidative stress in HFD mice.

Porelis downregulated adipogenic markers C/EBPα, PPAR-γ, and SREBP1 in treated mice.

Porelis inhibited pancreatic lipase activity in vitro with an IC50 of 223.3 µg/ml.

Abstract

Purple tea variety of Camelia sinensis (L.) Kuntze (Theaceae), with its unique presence of anthocyanins and 1,2-di-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl-β-D-glucose (GHG), has enormous potential in the weight management. Here, the weight loss mechanism of a standardized purple tea hydroalcoholic extract (PorelisTM, 3-5% GHG) is demonstrated in high-fat-diet-induced mice.

Male C57BL/6 mice (20±2 g) were divided into five groups (n=8). In a 12-week study, the control group was given a normal diet, and the other groups were given HFD (60 % kcal from fat) for 12 weeks. The treatment groups received Porelis at 25, 50, and 100 mg/kg body weight, PO.

Porelis treatment markedly reduced the body weight, fat pad weights, hepatic lipid accumulation, and oxidative stress in the HFD mice. The adipogenic markers, CCAAT/enhancer binding protein alpha (C/EBPα), Peroxisome proliferator-activated receptor gamma (PPAR-γ), and Sterol Regulatory Element Binding Protein 1 (SREBP1) were down-regulated in Porelis-treated mice compared to HFD model group (P<0.0001). Porelis treatment reduced the serum leptin level (P<0.0001) while increasing the adiponectin (P<0.01) in HFD mice. The circulating trimethylamine N-oxide (TMAO) (P<0.0001) and insulin (P<0.001) levels were significantly reduced in the Porelis groups compared to the HFD-alone group. In addition, Porelis markedly inhibited the in vitro pancreatic lipase activity (IC50 = 223.3 µg/ml).

Porelis exerts antiobesity activity in mice by regulating adipogenesis and energy homeostasis. Our data encourages further validation of Porelis-mediated weight loss effects in human subjects.

Figures