# Yeast gene of unknown function YGL081W is involved in DNA damage response

## TL;DR

This study identifies a yeast gene, YGL081W, as playing a role in the DNA damage response based on its domain structure and experimental results.

## Contribution

The study reveals a new role for the previously uncharacterized gene YGL081W in DNA damage response in yeast.

## Key findings

- YGL081W contains an FHA domain associated with DNA repair and cell cycle regulation.
- YGL081W knockout cells showed reduced growth under UV stress but not hydroxyurea.
- The gene's expression changes during mitotic stress, suggesting a stress response role.

## Abstract

This study aimed to better characterize gene of unknown function
YGL081W
in
Saccharomyces cerevisiae.
Bioinformatic analysis revealed that
YGL081W
possesses a forkhead associated (FHA) domain, known to be involved in DNA repair and cell cycle arrest. Furthermore,
YGL081W
was predicted to interact with genes involved in cell cycle regulation and its expression changed during mitotic stress.
YGL081W
knockout cells grew modestly better than wild type under normal growth conditions, grew significantly less than wild type when exposed to UV light, and grew no differently than wild type in the presence of hydroxyurea. Collectively, these data suggest
YGL081W
is involved in mediating the DNA damage response in
S. cerevisiae.