# Osteogenic potential of nanostructured Cu/W/Co in Fe-Mn alloys designed for maxillofacial applications: in vivo study in dog model

## TL;DR

This study tests biodegradable metal alloys in dogs to see if they help regenerate bone in maxillofacial areas, finding that adding Cu/W/Co improves bone healing.

## Contribution

The study introduces Cu/W/Co-doped Fe-Mn alloys with enhanced osteogenic potential for biodegradable maxillofacial implants.

## Key findings

- Alloys with Cu/W/Co showed significantly higher bone area and maturation compared to controls.
- FeMn32Co3 (M3) had the highest bone regeneration among tested alloys.
- Biodegradable alloys improved bone regeneration and reduced the need for implant removal surgeries.

## Abstract

To investigate osteogenic potential of biodegradable nanostructured Cu/W/Co in Fe-Mn alloys for maxillofacial applications in an in vivo model. Nanostructured FeMn35, FeMn32Cu3, FeMn32W3, FeMn32Co3 alloys were fabricated. Ten mongrel dogs were included where five mandibular defects were induced in each dog. Defects were randomly allocated into 5 groups ((M) control defects covered by bone disc, (M0) implanted by FeMn35 alloy, (M1) implanted by FeMn32Cu3 alloy, (M2) implanted by FeMn32W3 alloy, (M3) implanted by FeMn32Co3 alloy). Dogs were euthanized at 12 weeks for cone beam computed tomography, histologic and immunohistologic evaluation, and gene expression of osteopontin and osteocalcin. Defects implanted with metal demonstrated thicker bone trabeculae mixed with lamellar bone while control defects (M) demonestrated immature woven bone. Quantitative evaluation of bone area %, area % of mature bone and expression of osteopentin and osteocalcin bone markers revealed a statistically significant highest bone area % and maturation in group M3 compared to M2, M1, M0, and M group. A statistically significant increase in bone area % and maturation was recorded in M2 group compared to M1, M0, and M groups. A significantly increased bone area % and maturation was recorded in M1 compared to control M group and also between M0 and M group. Incorporating Cu/W/Co into Fe-Mn alloys enhanced biocompatibility and improved bone regeneration suggesting its suitability for use in various orthopedic and dental applications. Biodegradable metal alloys could improve patient outcome, reduce the need for additional surgeries to remove nonbiodegradble implants. Biodegradable metal alloys could improve patient outcome, reduce the need for additional surgeries to remove nonbiodegradble implants.