# Hematopoietic effects of Fufang E’jiao Jiang revealed by microbiome, metabolome and transcriptome analyses: a multi-omics strategy

## TL;DR

This study explores how Fufang E'jiao Jiang treats anemia in mice using multiple biological analyses.

## Contribution

The novel use of multi-omics analyses reveals FEJ's effects on anemia through microbiome, metabolome, and transcriptome interactions.

## Key findings

- FEJ alleviates anemia symptoms in mice induced by cyclophosphamide and acetylphenylhydrazine.
- FEJ improves gut microbiome imbalance by inhibiting harmful bacteria like Turicibacter and Akkermansia.
- FEJ regulates metabolic disorders linked to L-leucine, glycine, and other metabolites, and affects hematopoietic pathways.

## Abstract

Fufang E'jiao Jiang has been extensively utilized to replenish qi and nourish blood as the homology of medicine and food.

We analyzed the effects of FEJ on cyclophosphamide and acetylphenylhydrazine-induced anemia mice through gut microbiome analysis, fecal metabolomics, and transcriptome sequencing.

FEJ markedly alleviated the anemia symptoms in the mice. FEJ markedly alleviated the anemia symptoms caused by cyclophosphamide and acetylphenylhydrazine induction. FEJ improved the gut microbiome imbalance by inhibiting the proliferation of harmful bacteria Turicibacter, Akkermansia and Tuzzerella. Fecal metabolomic data showed that FEJ regulated metabolic disorders in anemia mice and was probably associated with L-leucine, L-proline, glycine, phenylalanine, propanoic acid and butanoic acid. Transcriptome analysis indicated the amelioration of anemia was predominantly associated with the hematopoietic cell lineage, osteoclast formation and B cell receptor signaling pathway. According to Spearman's correlation analysis, there was a strong link between gut microbiota and hematopoietic index, metabolites and genes.

Our study supports the application of FEJ in anemia treatment.