# Evaluation of the Protective Effects of Moringa oleifera, Zingiber officinale, and Allium sativum in a Rat Model of Chronic Kidney Disease (CKD)

## TL;DR

This study tests if a mix of Moringa, ginger, and garlic can protect against kidney disease in rats by reducing oxidative stress and tissue damage.

## Contribution

The novel contribution is evaluating a combined polyherbal extract's protective effects against CKD in a rat model.

## Key findings

- Polyherbal extract significantly improved kidney function markers like creatinine and blood urea nitrogen.
- The extract reduced oxidative stress and fibrosis in kidney tissues.
- Higher doses of the extract showed more pronounced protective effects.

## Abstract

Background: Oxidative stress plays a key role in the development and progression of chronic kidney disease (CKD). In this study, it has been hypothesized that the extract of Moringa oleifera leaves, Zingiber officinale rhizomes, and Allium sativum cloves together might exert a protective effect against adenine-induced CKD in rats.

Methods: Rats were randomly divided into five groups: Group 1: normal control; Group 2: disease control; Group 3: CKD + polyherbal extract (250 mg/kg); Group 4: CKD + polyherbal extract (500 mg/kg); Group 5: CKD + polyherbal extract (1500 mg/kg). CKD was induced by feeding rats a 0.3% adenine-mixed diet for 28 days. Polyherbal extract was administered to rats, except for Groups 1 and 2, for 28 days.

Results: Experimental animals receiving treatment with polyherbal extract showed significant improvement in water intake (p< 0.001), urine volume (p< 0.01), urine creatinine (p < 0.05), plasma creatinine (p < 0.01), and blood urea nitrogen(p<0.05). Furthermore, the combined extract showed a potential effect in reducing oxidative stress and fibrosis, indicated by reduced hypertrophy in kidney tissue.

Conclusion: Polyherbal extract containing Moringa oleifera leaves, Zingiber officinale rhizomes, and Allium sativum clove extracts showed a reduction in fibrosis and oxidative stress, and thus, this polyherbal extract combination could be explored in-depth for its therapeutic potential in CKD.