# Genome Editing of the NF-YA8 Gene Modifies Tomato Plant Architecture and Fruit Traits

## TL;DR

Editing the NF-YA8 gene in tomatoes changes plant structure and fruit traits, offering a way to improve tomato varieties.

## Contribution

This study demonstrates that non-transgenic genome editing of NF-YA8 in tomatoes alters key agronomic traits.

## Key findings

- Mutations in NF-YA8 led to changes in plant architecture and fruit characteristics.
- Phenotypic changes included altered cotyledon development, stem structure, and flowering time.
- NF-YA8 functions as a high-level regulator of developmental processes in tomato plants.

## Abstract

Genome editing has revolutionized plant science, providing an unprecedented ability to precisely manipulate plant genomes. For this study, genome editing was utilized to target and modify the NF-YA8 transcription factor (TF) in tomato plants (Solanum lycopersicum L. var. Heinz 1706). The primary objective of this research was to introduce targeted mutations in a non-transgenic manner to the NF-YA8 gene, which encodes the alpha subunit of the Nuclear Factor-Y (NF-Y) heterotrimeric TF, and explore its potential for developing new and improved tomato varieties. Through the transient expression of custom-engineered zinc finger nucleases (ZFNs) in tomato seeds, mutations were successfully introduced in the target gene. The recovered mutant NF-YA8 coding sequences showed a significant level of similarity to the wild type, with a range of 86.9% to 98.21%. Genotyping M2 lines revealed monogenic mutations at or near the intended target site. Phenotypic changes were also evident in both vegetative and reproductive stages of plants. The research revealed that NF-YA8 functions as a high-level regulator, orchestrating a developmental cascade that influences key agronomic traits throughout the plant’s life cycle, including cotyledon development, stem architecture, inflorescence architecture, flowering time, and fruit size and shape.