# Establishment and Partial Characterization of Three Novel Permanent Cell Lines Originating from European Freshwater Fish Species

## TL;DR

Researchers created three new cell lines from European freshwater fish that can help diagnose and study viral diseases in these species.

## Contribution

Three novel continuous cell lines from European freshwater fish species were established and characterized for viral susceptibility.

## Key findings

- The cell lines NPL-3, AF-1, and PF-1 have been subcultured over 90 times and maintained in M199 medium at 25 °C.
- NPL-3 and AF-1 cells are susceptible to multiple viruses, while PF-1 cells support only two of them.
- The cell lines could be used for diagnosing viral diseases and isolating new viruses in freshwater fish.

## Abstract

The establishment and partial characterization of three continuous cell lines from European freshwater fish species are provided. The three new cell lines, designated NPL-3, AF-1, and PF-1, were created from larvae of northern pike (Esox lucius) and fin tissues of asp (Leuciscus aspius) and European perch (Perca fluviatilis) fin tissues, respectively. All three cell lines have been subcultured more than 90 times since their establishment. Cells were optimally maintained at 25 °C in M199 medium supplemented with 10% fetal bovine serum. The NPL-3 and AF-1 cells are susceptible to spring viraemia of carp virus, pike fry rhabdovirus, ictalurid herpesvirus 2, and European catfish virus, while in the PF-1 cells, only the latter two viruses were successfully propagated. These newly established cell lines could serve as diagnostic tools for the aforementioned economically important viral diseases. They might be effective appliances for isolating novel viruses from northern pike, asp, European perch, and other closely related fish species.