# New Terpenoids and Polyphenolic Profile of Carpesium cernuum L. of European Origin

## TL;DR

This study identifies new compounds in Carpesium cernuum from Europe and tests one for neuroprotective and cytotoxic effects.

## Contribution

The discovery of three new terpenoids and a polyphenolic profile in European Carpesium cernuum is novel.

## Key findings

- Three new terpenoids and a dihydrobenzofuran derivative were isolated from Carpesium cernuum.
- The polyphenolic profile includes 24 hydroxycinnamates dominated by caffeoylquinic and caffeoylhexaric acids.
- A monoterpenoid derivative showed partial neuroprotection and cytotoxic effects in SH-SY5Y cells.

## Abstract

Carpesium cernuum L., the most widespread representative of the genus Carpesium, has been traditionally used in some regions of Asia as a remedy for various ailments or as a vegetable. Although the plant is distributed in Europe, there is no data on its medicinal use in this part of the world. The chemical composition of European Carpesium cernuum L. has remained unknown until now, except for the compositions of essential oils distilled from the roots and aerial parts of the plant. Polyphenolic profiles of hydroalcoholic extracts from C. cernuum were studied using the HPLC-MSn technique. The analysis revealed the presence of 24 hydroxycinnamates, which were dominated by caffeoylquinic and caffeoylhexaric acids. Moreover, fractionation of the chloroform extracts from the plant led to the isolation of three new compounds, 8α-angeloyloxy-4β-hydroxy-5β-(3-methylbutyryloxy)-9-oxo-germacran-6α,12-olide, 9β-angeloyloxy-4β,8α-dihydroxy-5β-(3-methylbutyryloxy)-3-oxo-germacran-6α,12-olide, and a dihydrobenzofuran derivative, together with twelve known compounds. 8-Hydroxy-9,10-diisobutyryloxythymol, a monoterpenoid thymol derivative from the roots of the plant, was evaluated for potential neuroprotective and cytotoxic activities using differentiated and undifferentiated SH-SY5Y neuroblastoma cells. At a concentration range of 1–10 μM, the compound provided partial (up to 50%) protection against H2O2-induced cell damage in the undifferentiated cells. At concentrations higher than 25 μM, the monoterpenoid significantly reduced the viability of the cells (IC50: 65.7 μM for the undifferentiated cells and 40.9 μM for the differentiated cells).